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Immobilization of biomolecules on cysteamine-modified polyaniline film for highly sensitive biosensing

Cai, Q. and Xu, B. and Ye, L. and Zengfeng, Z. and Zhang, J. and Jin, Q. and Zhao, J. and Xue, J. and Chen, X. (2013) Immobilization of biomolecules on cysteamine-modified polyaniline film for highly sensitive biosensing. Talanta, 120. pp. 462-469. DOI: 10.1016/j.talanta.2013.11.013

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Abstract

We present a new cysteamine (CS)-modified polyaniline (PANI) film for highly efficient immobilization of biomolecules in biosensing technology. This electrochemical deposited PANI film treated with CS and glutaraldehyde could be employed as an excellent substrate for biomolecules immobilization. The parameters of PANI growth were optimized to obtain suitable surface morphology of films for biomolecules combination with the help of electron and atomic force microscopy. Cyclic voltammetry (CV) was utilized to illustrate the different electrochemical activities of each modified electrode. Due to the existence of sulfydryl group and amino group in CS, surface modification with CS was proven to reduce oxidized units on PANI film remarkably, as evidenced by both ATR-FTIR and Raman spectroscopy characterizations. Furthermore, bovine serum albumin (BSA) was used as the model protein to investigate the immobilization efficiency of biomolecules on the PANI film, comparative study using quartz crystal microbalance (QCM) showed that BSA immobilized on CS-modified PANI could be increased by at least 20% than that without CS-modified PANI in BSA solution with the concentration of 0.1�1 mg/mL. The CS-modified PANI film would be significant for the immobilization and detection of biomolecules and especially promising in the application of immunosensor for ultrasensitive detection.

Item Type: Article
Subjects: Research Publications
Departments: College of Physical and Applied Sciences > School of Electronic Engineering
Date Deposited: 02 Apr 2016 03:14
Last Modified: 02 Apr 2016 03:14
ISSN: 0039-9140
URI: http://e.bangor.ac.uk/id/eprint/6448
Identification Number: DOI: 10.1016/j.talanta.2013.11.013
Publisher: Elsevier
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