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EDC-Mediated Oligonucleotide Immobilization on a Long Period Grating Optical Biosensor

Chen, X. and Liu, C. and Hughes, M. and Nage, D.A. and Hine, A.V. and Zhang, L. (2015) EDC-Mediated Oligonucleotide Immobilization on a Long Period Grating Optical Biosensor. Journal of Biosensors & Bioelectronics, 6 (2). DOI: 10.4172/2155-6210.1000173

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Abstract

e present the development and simplification of label-free fiber optic biosensors based on immobilization of oligonucleotides on dual-peak long period gratings (dLPGs). This improvement is the result of a simplification of biofunctionalization methodology. A one-step 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated reaction has been developed for the straightforward immobilization of unmodified oligonucleotides on the glass fiber surface along the grating region, leading to covalent attachment of a 5'-phosphorylated probe oligonucleotide to the amino-derivatized fiber grating surface. Immobilization is achieved via a 5'phosphate-specific linkage, leaving the remainder of the oligonucleotide accessible for binding reactions. The dLPG has been tested in different external media to demonstrate its inherent ultrahigh sensitivity to the surrounding-medium refractive index (RI) achieving 50-fold improvement in RI sensitivity over the previously-published LPG sensor in media with RI's relevant to biological assays. After functionalization, the dLPG biosensor was used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM. The proposed one-step EDC reaction approach can be further extended to develop fiber optic biosensors for disease analysis and medical diagnosis with the advances of label-free, real-time, multiplex, high sensitivity and specificity.

Item Type: Article
Subjects: Research Publications
Departments: College of Physical and Applied Sciences > School of Electronic Engineering
Date Deposited: 02 Apr 2016 03:13
Last Modified: 02 Apr 2016 03:13
ISSN: 2155-6210
URI: http://e.bangor.ac.uk/id/eprint/6433
Identification Number: DOI: 10.4172/2155-6210.1000173
Publisher: Omics International
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