In Vitro Evaluation of the Interaction of Dextrin-Colistin Conjugates with Bacterial Lipopolysaccharide

Roberts, J.L. and Cattoz, B. and Schweins, R. and Beck, K. and Thomas, D.W. and Griffiths, P.C. and Ferguson, E.L. (2016) In Vitro Evaluation of the Interaction of Dextrin-Colistin Conjugates with Bacterial Lipopolysaccharide. Journal of Medicinal Chemistry, 59 (2). pp. 647-654. DOI: 10.1021/acs.jmedchem.5b01521

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Dextrin-colistin conjugates have been developed with the aim of achieving reduced clinical toxicity associated with colistin, also known as polymyxin E, and improved targeting to sites of bacterial infection. This study investigated the in vitro ability of such dextrin-colistin conjugates to bind and modulate bacterial lipopolysaccharide (LPS), and how this binding affects its biological activity. These results showed that colistin and amylase-activated dextrin-colistin conjugate to a lesser extent induced aggregation of LPS to form a stacked bilayer structure with characteristic dimensions, although this did not cause any substantial change in its secondary structure. In biological studies, both colistin and dextrin-colistin conjugate effectively inhibited LPS-induced hemolysis and tumor necrosis factor alpha (TNF alpha) secretion in a concentration-dependent manner, but only dextrin-colistin conjugate showed no additive toxicity at higher concentrations. This study provides the first direct structural experimental evidence for the binding of dextrin-colistin conjugates and LPS and gives insight into the mode of action of dextrin-colistin conjugates.

Item Type: Article
Subjects: Research Publications
Departments: College of Health and Behavioural Sciences > North Wales Centre for Primary Care Research
Date Deposited: 02 Mar 2016 03:28
Last Modified: 02 Mar 2016 03:28
ISSN: 0022-2623
URI: http://e.bangor.ac.uk/id/eprint/6276
Identification Number: DOI: 10.1021/acs.jmedchem.5b01521
Publisher: American Chemical Society
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