Detection of covalent DNA-bound Spo11 and topoisomerase complexes

Hartsuiker, E. (2011) Detection of covalent DNA-bound Spo11 and topoisomerase complexes. In: DNA recombination: Methods and Protocols. Springer Science and Business Media LLC, pp. 65-77. ISBN 9781617791291

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Topoisomerases can release topological stress and resolve DNA catenanes by a DNA strand breakage and re-ligation mechanism. During the lifetime of the DNA break, the topoisomerase remains covalently linked to the DNA and removes itself when the break is re-ligated. While the lifetime of a covalent topoisomerase-DNA complex is usually short, several clinically important cancer drugs kill cancer cells by inhibiting the removal of covalently linked topoisomerases. The topoisomerase-like protein Spo11 is responsible for meiotic double strand break formation. Spo11 is not able to remove itself and is removed by nucleolytic cleavage. This chapter describes a method which allows the reproducible and quantitative detection of proteins covalently bound to the DNA

Item Type: Book Section
Subjects: Research Publications
Departments: College of Health and Behavioural Sciences > School of Medical Sciences
Date Deposited: 17 Dec 2015 03:23
Last Modified: 17 Dec 2015 03:23
ISBN: 9781617791291
URI: http://e.bangor.ac.uk/id/eprint/5979
Identification Number: DOI: 10.1007/978-1-61779-129-1_5
Publisher: Springer Science and Business Media LLC
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