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Primary skeletal muscle cells cultured on gelatin bead microcarriers develop structural and biochemical features characteristic of adult skeletal muscle

Kubis, H-P. and Scheibe, R.J. and Decker, B. and Hufendiek, K. and Hanke, N. and Gros, G and Meissner, J.D. (2016) Primary skeletal muscle cells cultured on gelatin bead microcarriers develop structural and biochemical features characteristic of adult skeletal muscle. Cell Biology International. DOI: 10.1002/cbin.10565

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Abstract

A primary skeletal muscle cell culture, in which myoblasts derived from newborn rabbit hindlimb muscles grow on gelatin bead microcarriers in suspension and differentiate into myotubes, has been established previously. In the course of differentiation and beginning spontaneous contractions, these multinucleated myotubes do not detach from their support. Here, we describe the development of the primary myotubes with respect to their ultrastructural differentiation. Scanning electron microscopy reveals that myotubes not only grow around the surface of one carrier bead but also attach themselves to neighbouring carriers, forming bridges between carriers. Transmission electron microscopy demonstrates highly ordered myofibrils, T-tubules and sarcoplasmic reticulum. The functionality of the contractile apparatus is evidenced by contractile activity that occurs spontaneously or can be elicited by electrostimulation. Creatine kinase activity increases steadily until day 20 of culture. Regarding the expression of isoforms of myosin heavy chains (MHC), we could demonstrate that from day 16 on, no non-adult MHC isoform mRNAs are present. Instead, on day 28 the myotubes express predominantly adult fast MHCIId/x mRNA and protein. This MHC pattern resembles that of fast muscles of adult rabbits. In contrast, primary myotubes grown on matrigel-covered culture dishes express substantial amounts of non-adult MHC protein even on day 21. To conclude, primary myotubes grown on microcarriers in their later stages exhibit many features of adult skeletal muscle and characteristics of fast type II fibers. Thus, the culture represents an excellent model of adult fast skeletal muscle, for example when investigating molecular mechanisms of fast-to-slow fiber type transformation

Item Type: Article
Subjects: Research Publications
Departments: College of Health and Behavioural Sciences > School of Sport, Health and Exercise Sciences
Date Deposited: 11 Dec 2015 03:25
Last Modified: 20 Jan 2017 03:14
ISSN: 1065-6995
Publisher's Statement: "This is the peer reviewed version of the following article: 'Primary skeletal muscle cells cultured on gelatin bead microcarriers develop structural and biochemical features characteristic of adult skeletal muscle by Kubis, H-P.; Scheibe, R.J.; Decker, B.; Hufendiek, K.; Hanke, N.; Gros, G,; & Meissner, J.D.' which has been published in final form at DOI: 10.1002/cbin.10565. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
URI: http://e.bangor.ac.uk/id/eprint/5964
Identification Number: DOI: 10.1002/cbin.10565
Publisher: Wiley
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