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Detection of Uranium Contamination in Acacia Cell Sap by Capillary Zone Electrophoresis (CZE) Technique

Al-Salih, H.S. and Fathi, R.A. and Godbold, D.L. and Tomor, A.D. (2014) Detection of Uranium Contamination in Acacia Cell Sap by Capillary Zone Electrophoresis (CZE) Technique. Journal of Natural Science Research, 4 (6). pp. 40-48.

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Abstract

The study was carried out to detect uranium level in the cell sap of acacia plant cells, for this purpose callus cultures of Acacia albida was used as well as plants. Cell saps from both callus and plant leaf were taken using Micro capillary syringe and detected using Capillary zone electrophoresis technique. It was shown that using citrate buffer of 3.0pH help in detecting uranium accumulated in the cells. Prospective calculation for the level of uranium uptake showed that 1.64mM is the level of uranium in the leaf cells that had been grown on soil with addition of 500 mg.kg-1 uranyl nitrate for 3 months, while for callus which grown on MS medium with addition of 500 mg/l uranyl nitrate for the same time (3 months), uptake of uranium reached 0.8 mM. The comparison between TXRF analysis of uranium accumulated in plant tissues and CZE efficiency in detecting uranium level in cell sap of both leaves and callus cells, it was shown that both techniques prove that A.albida plants can accumulate uranium with a level double as that taken by callus cells.

Item Type: Article
Subjects: Research Publications
Departments: College of Natural Sciences > School of Biological Sciences
Date Deposited: 25 Nov 2015 03:19
Last Modified: 04 Dec 2015 03:32
ISSN: 2224-3186
URI: http://e.bangor.ac.uk/id/eprint/5908
Publisher: IISTE
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