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Metaproteomics and metabolomics analyses of chronically petroleum-polluted sites reveal the importance of general anaerobic processes uncoupled with degradation

Bargiela, R. and Herbst, F.A. and Martinez-Martinez, M. and Seifert, J. and Rojo, D. and Cappello, S. and Genovese, M. and Crisafi, F. and Denaro, R. and Chernikova, T.N. and Barbas, C. and von Bergen, M. and Yakimov, M.M. and Ferrer, M. and Golyshin, P.N. (2015) Metaproteomics and metabolomics analyses of chronically petroleum-polluted sites reveal the importance of general anaerobic processes uncoupled with degradation. Proteomics, 15 (20). pp. 3508-3520. DOI: 10.1002/pmic.201400614

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Abstract

Crude oil is one of the most important natural assets for humankind, yet it is a major environmental pollutant, notably in marine environments. One of the largest crude oil polluted areas in the word is the semi-enclosed Mediterranean Sea, in which the metabolic potential of indigenous microbial populations towards the large-scale chronic pollution is yet to be defined, particularly in anaerobic and micro-aerophilic sites. Here, we provide an insight into the microbial metabolism in sediments from three chronically polluted marine sites along the coastline of Italy: the Priolo oil terminal/refinery site (near Siracuse, Sicily), harbour of Messina (Sicily) and shipwreck of MT Haven (near Genoa). Using shotgun metaproteomics and community metabolomics approaches, the presence of 651 microbial proteins and 4776 metabolite mass features have been detected in these three environments, revealing a high metabolic heterogeneity between the investigated sites. The proteomes displayed the prevalence of anaerobic metabolisms that were not directly related with petroleum biodegradation, indicating that in the absence of oxygen, biodegradation is significantly suppressed. This suppression was also suggested by examining the metabolome patterns. The proteome analysis further highlighted the metabolic coupling between methylotrophs and sulphate reducers in oxygen-depleted petroleum-polluted sediments.

Item Type: Article
Subjects: Research Publications
Departments: College of Natural Sciences > School of Biological Sciences
Date Deposited: 25 Nov 2015 03:18
Last Modified: 25 Nov 2015 09:54
ISSN: 1615-9853
URI: http://e.bangor.ac.uk/id/eprint/5901
Identification Number: DOI: 10.1002/pmic.201400614
Publisher: Wiley VCH
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