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Botulinum Neurotoxin Serotypes Detected by Electrochemical Impedance Spectroscopy

Savage, A.C. and Buckley, N. and Halliwell, J. and Gwenin, C.D. (2015) Botulinum Neurotoxin Serotypes Detected by Electrochemical Impedance Spectroscopy. Toxins, 7. pp. 1544-1555. DOI: 10.3390/toxins7051544

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Abstract

Botulinum neurotoxin is one of the deadliest biological toxins known to mankind and is able to cause the debilitating disease botulism. The rapid detection of the different serotypes of botulinum neurotoxin is essential for both diagnosis of botulism and identifying the presence of toxin in potential cases of terrorism and food contamination. The modes of action of botulinum neurotoxins are well-established in literature and differ for each serotype. The toxins are known to specifically cleave portions of the SNARE proteins SNAP-25 or VAMP; an interaction that can be monitored by electrochemical impedance spectroscopy. This study presents a SNAP-25 and a VAMP biosensors for detecting the activity of five botulinum neurotoxin serotypes (A-E) using electrochemical impedance spectroscopy. The biosensors are able to detect concentrations of toxins as low as 25 fg/mL, in a short time-frame compared with the current standard methods of detection. Both biosensors show greater specificity for their compatible serotypes compared with incompatible serotypes and denatured toxins.

Item Type: Article
Subjects: Research Publications
Departments: College of Physical and Applied Sciences > School of Chemistry
Date Deposited: 20 May 2015 02:49
Last Modified: 17 Oct 2015 02:39
ISSN: 2072-6651
Publisher's Statement: Published by MDPI AG, Basel, Switzerland
URI: http://e.bangor.ac.uk/id/eprint/4563
Identification Number: DOI: 10.3390/toxins7051544
Publisher: MDPI AG
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